Table 1. Bacterial strains and plasmids.
| Strain or plasmid | Relevant phenotype, genotype or description | Source or reference |
|---|---|---|
| K.oxytoca | ||
| M5a1 | R+KpnAI M+KpnAI | (13) |
| M5a1R | R−KpnAI M+KpnAI | (14) |
| Salmonella–E.coli hybrids | ||
| L4039 | R+SEAI M+SEAI | (16) |
| L4030 | R+SENI M+SENI | (16) |
| L4039 | R+SGI M+SGI | (16) |
| L4021 | R+STI M+STI | (16) |
| E.coli | ||
| DH5α | R−K M+K | Lab stock |
| Plasmids | ||
| Lambda subclonesa | ||
| pL1 (5.5 kb) | BamHI clone of phage lambda | (11) |
| pL2 (16.8 kb) | BamHI clone of phage lambda | (11) |
| pL3 (5.6 kb) | BamHI clone of phage lambda | (11) |
| pL4 (6.5 kb) | BamHI clone of phage lambda | (11) |
| pL5 (7.2 kb) | BamHI clone of phage lambda | (11) |
| pL6 (6.8 kb) | BamHI clone of phage lambda | (11) |
| pL7 (3.4 kb) | Subclone of pL1 | This study |
| pL8 (6.5 kb) | Subclone of pL2 | This study |
| pL9 (10.3 kb) | Subclone of pL2 | This study |
| pL10 (3.8 kb) | Subclone of pL4 | This study |
| pL11 (1.4 kb) | Subclone of pL7 | This study |
| pL12 (3.1 kb) | Subclone of pL8 | This study |
| pL13 (1.1 kb) | Subclone of pL9 | This study |
| pL14 (0.13 kb) | Subclone of pL11 | This study |
| pL15 (3.7 kb) | EcoRV clone of phage lambda | This study |
| pL16 (2.0 kb) | HindIII clone of phage lambda | This study |
| pL17 (2.3 kb) | HindIII clone of phage lambda | This study |
| pL18 (0.56 kb) | HindIII clone of phage lambda | This study |
| pL19 (6.6 kb) | HindIII clone of phage lambda | This study |
| pL20 (1.6 kb) | Subclone of pL19 | This study |
| pL21 (0.77 kb) | Subclone of pL20 | This study |
| pL26 (4.9 kb) | EcoRI clone of phage lambda | This study |
| pL27 (5.6 kb) | EcoRI clone of phage lambda | This study |
| pL28 (5.8 kb) | EcoRI clone of phage lambda | This study |
| pL29 (3.9 kb) | EcoRV clone of phage lambda | This study |
| pL30 (1.7 kb) | EcoRV clone of phage lambda | This study |
| E.coli subclonesa | E.coli map coordinate | (19) |
| pE2 (5.8 kb) | 1557431-1563188 | This study |
| pE3 (2.8 kb) | 1608569-1611375 | This study |
| pE4 (4.1 kb) | 1565001-1569077 | This study |
| pE5 (11.4 kb) | 1593721-1605160 | This study |
| pE6 (8.1 kb) | 1402492-1410639 | This study |
| pE8 (12.9 kb) | 1505286-1518231 | This study |
| pE9 (1.7 kb) | 1523044-1524690 | This study |
| pE10 (1.9 kb) | 1582185-1584064 | This study |
| pE11 (3.0 kb) | 1465811-1468761 | This study |
| pE12 (5.1 kb) | 1479912-1485078 | This study |
| pE14 (4.4 kb) | 1553017-1557430 | This study |
| pE15 (4.8 kb) | 1518232-1523043 | This study |
| pE16 (4.5 kb) | 1426716-1431168 | This study |
| pE17 (3.2 kb) | 1584065-1587223 | This study |
| pE18 (6.5 kb) | 1524691-1593720 | This study |
| pE19 (4.9 kb) | 1468762-1473696 | This study |
| pE22 (6.2 kb) | 1473697-1479911 | This study |
| pE23 (2.6 kb) | 1456295-1458942 | This study |
| pE24 (1.6 kb) | 1563189-1564830 | This study |
| pE26 (3.9 kb) | 1647821-1651757 | This study |
| pE28 (0.6 kb) | 1674657-1675295 | This study |
| pE29 (3.7 kb) | 1342364-1346037 | This study |
| pE31 (3.1 kb) | 1336254-1339389 | This study |
| pE33 (2.4 kb) | 1339984-1342363 | This study |
| pE38 (0.6 kb) | 1339390-1339983 | This study |
| pE44 (1.0 kb) | 1278264-1279262 | This study |
| pE45 (16.6 kb) | 1265543-1280035 | This study |
| Oligonucleotide clones | ||
| pKpnAI | Plasmid with KpnAI site | This study |
| pKpnAI-H1 | Plasmid with KpnAI/HindIII sites | This study |
| pKpnAI-H2 | Plasmid with KpnAI/HindIII sites | This study |
| pKpnAI-H3 | Plasmid with KpnAI/HindIII sites | This study |
| pSEAI-S1 | Plasmid with SEAI/ScaI sites | This study |
| pSEAI-S2 | Plasmid with SEAI/ScaI sites | This study |
| pSENI-S1 | Plasmid with SENI/ScaI sites | This study |
| pSENI-S2 | Plasmid with SEAI/ScaI sites | This study |
| pSGI-S1 | Plasmid with SGI/SpeI sites | This study |
| pSGI-T1 | Plasmid with SGI/Tth111I sites | This study |
aLocations of lambda and E.coli subclones are shown in Figure 1.