Figure 3.

Strand cleavage inhibition is distinguishable from complex assembly inhibition. Tnp complexed with fluorescently labeled DNA exhibits a significantly higher FP value than either the free DNA substrate (mock complex assembly inhibition) or the cleaved donor DNA product (no inhibition). The reactions were performed as originally described in Figure 2 and in Materials and methods. Tnp storage buffer was added to the DNA substrate in place of Tnp for mock complex assembly inhibition. For catalytic inhibition, 10 mM EDTA was added to the cleavage reaction. The reactions were performed in triplicate.