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. 2015 Jan 23;7(2):219–237. doi: 10.3390/toxins7020219

Figure 1.

Figure 1

(A) Reverse-phase HPLC chromatogram of lyophilised Androctonus aeneas venom. The arrow indicates the elution position/retention time of the peptide fraction (#145) exhibiting antimicrobial activity. AU = 214 nm, and the gradient was formed from TFA/water (0.05:99.95, v/v) to TFA/water/acetonitrile (0.1:19.95:80.00, v/v/v) in 240 min at a flow rate of 1 mL/min. (B) MALDI-TOF mass spectrum of a sample of Fraction #145 indicating singly-charged [M + H]+ ions at m/z 1989.50 and m/z 2017.68. The ion at m/z 2039.07 is likely to be a sodium adduct ion [M + Na]+ of the peptide at m/z 2017.68.