Table 1.
Predicted singly- and doubly-charged b-ions and y-ions arising from MS/MS fragmentation of AaeAP1. Ions observed following MS/MS fragmentation are indicated in bold typeface and are underlined.
| #1 | b(1+) | b(2+) | Sequence | y(1+) | y(2+) | #2 |
|---|---|---|---|---|---|---|
| 1 | 148.07570 | 74.54149 | F | - | - | 19 |
| 2 | 261.15977 | 131.08352 | L | 1869.13213 | 935.06970 | 18 |
| 3 | 408.22819 | 204.61773 | F | 1756.04806 | 878.52767 | 17 |
| 4 | 495.26022 | 248.13375 | S | 1608.97964 | 804.99346 | 16 |
| 5 | 608.34429 | 304.67578 | L | 1521.94761 | 761.47744 | 15 |
| 6 | 721.42836 | 361.21782 | I | 1408.86354 | 704.93541 | 14 |
| 7 | 818.48113 | 409.74420 | P | 1295.77947 | 648.39337 | 13 |
| 8 | 905.51316 | 453.26022 | S | 1198.72670 | 599.86699 | 12 |
| 9 | 1004.58158 | 502.79443 | V | 1111.69467 | 556.35097 | 11 |
| 10 | 1117.66565 | 559.33646 | I | 1012.62625 | 506.81676 | 10 |
| 11 | 1188.70277 | 594.85502 | A | 899.54218 | 450.27473 | 9 |
| 12 | 1245.72424 | 623.36576 | G | 828.50506 | 414.75617 | 8 |
| 13 | 1358.80831 | 679.90779 | L | 771.48359 | 386.24543 | 7 |
| 14 | 1457.87673 | 729.44200 | V | 658.39952 | 329.70340 | 6 |
| 15 | 1544.90876 | 772.95802 | S | 559.33110 | 280.16919 | 5 |
| 16 | 1615.94588 | 808.47658 | A | 472.29907 | 236.65317 | 4 |
| 17 | 1729.02995 | 865.01861 | I | 401.26195 | 201.13461 | 3 |
| 18 | 1885.13107 | 943.06917 | R | 288.17788 | 144.59258 | 2 |
| 19 | - | - | C-Amidated | 132.07676 | 66.54202 | 1 |