Table 2.
Predicted singly- and doubly-charged b-ions and y-ions arising from MS/MS fragmentation of AaeAP2. Ions observed following MS/MS fragmentation are indicated in bold typeface and are underlined.
| #1 | b(1+) | b(2+) | Sequence | y(1+) | y(2+) | #2 |
|---|---|---|---|---|---|---|
| 1 | 148.07570 | 74.54149 | F | - | - | 19 |
| 2 | 261.15977 | 131.08352 | L | 1841.10083 | 921.05405 | 18 |
| 3 | 408.22819 | 204.61773 | F | 1728.01676 | 864.51202 | 17 |
| 4 | 495.26022 | 248.13375 | S | 1580.94834 | 790.97781 | 16 |
| 5 | 608.34429 | 304.67578 | L | 1493.91631 | 747.46179 | 15 |
| 6 | 721.42836 | 361.21782 | I | 1380.83224 | 690.91976 | 14 |
| 7 | 818.48113 | 409.74420 | P | 1267.74817 | 634.37772 | 13 |
| 8 | 905.51316 | 453.26022 | S | 1170.69540 | 585.85134 | 12 |
| 9 | 976.55028 | 488.77878 | A | 1083.66337 | 542.33532 | 11 |
| 10 | 1089.63435 | 545.32081 | I | 1012.62625 | 506.81676 | 10 |
| 11 | 1160.67147 | 580.83937 | A | 899.54218 | 450.27473 | 9 |
| 12 | 1217.69294 | 609.35011 | G | 828.50506 | 414.75617 | 8 |
| 13 | 1330.77701 | 665.89214 | L | 771.48359 | 386.24543 | 7 |
| 14 | 1429.84543 | 715.42635 | V | 658.39952 | 329.70340 | 6 |
| 15 | 1516.87746 | 758.94237 | S | 559.33110 | 280.16919 | 5 |
| 16 | 1587.91458 | 794.46093 | A | 472.29907 | 236.65317 | 4 |
| 17 | 1700.99865 | 851.00296 | I | 401.26195 | 201.13461 | 3 |
| 18 | 1857.09977 | 929.05352 | R | 288.17788 | 144.59258 | 2 |
| 19 | - | - | C-Amidated | 132.07676 | 66.54202 | 1 |