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. 2015 Jan 27;7(2):238–254. doi: 10.3390/toxins7020238

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Dependences of optical density (OD) registered in the ELISA from the concentration of anti-AFB1 antibodies obtained for different methods of immune complex labeling. (A) variants of peroxidase conjugate binding (1—antibody-biotin + antispecies antibody—peroxidase, 2—antibody-biotin + streptavidin-peroxidase, 3—antibody-additional bridge-biotin + streptavidin-peroxidase); (B) variants of peroxidase conjugates (1—antispecies antibody—peroxidase, 2—streptavidin-polyperoxidase). AFB1-BSA conjugate was immobilized in microplate wells from 0.5 μg/mL; other parameters of the ELISA experiment are given in the Experimental Section (data are presented for three replicates).