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. 2015 Jan 27;7(2):238–254. doi: 10.3390/toxins7020238

Figure 2.

Figure 2

The proposed rapid ELISA format and the optimization sequence employed (1, polystyrene microplate; 2, immobilized mycotoxin–protein conjugate; 3, specific antibody–biotin conjugate; 4, mycotoxin in the tested sample; 5, streptavidin–polyperoxidase conjugate; stage A, competitive immunochemical interaction; stage B, interaction of specific complexes with streptavidin-polyperoxidase; stage C, enzymatic reaction).