. 2015 Jan 29;7(2):299–321. doi: 10.3390/toxins7020299

Table 2.

Bacterial strains and plasmids used in this study. Double colon (::) indicates fusion of a promoter and gene.

Strains	Genotype	Source
BW25113	rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1	[59]
BW25113 ∆ortT	BW25113 ΔortT Ω Km^R	[59]
BW25113 ∆ghoT ∆kan	BW25113 ΔghoT	[42]
BW25113 ∆ortT ∆kan	BW25113 ΔortT	This study
BW25113 ∆mqsR ∆mqsA ∆kan	BW25113 ∆mqsR ∆mqsA	[43]
Plasmids	Genotype	Source
pCA24N	Cm^R; lacI^q	[60]
pCA24N-ghoT	Cm^R; lacI^q, P_T5-lac::ghoT⁺	[60]
pCA24N-ortT	Cm^R; lacI^q, P_T5-lac::ortT⁺	[60]
pCA24N-relA	Cm^R; lacI^q, P_T5-lac::relA⁺	[60]
pCA24N-ortTACG	Cm^R; lacI^q, P_T5-lac::ortT⁺ with start codon ATG replaced with ACG	This study
pCA24N-ortTF38R	Cm^R; lacI^q, P_T5-lac::ortT⁺ with TT changed to CG at nucleotide position 160-161 relative to the 6xHis-GhoT start codon	This study
pCA24N-ghoTF38R	Cm^R; lacI^q, pCA24N P_T5-lac::ghoT⁺ with TT changed to CG at nucleotide position 160-161 relative to the 6xHis-GhoT start codon	[42]
pCA24N-ortT-ydcY	Cm^R; lacI^q, pCA24N P_T5-lac::ortT⁺-ydcY⁺	This study
pCA24N-ortT-ghoS	Cm^R; lacI^q, pCA24N P_T5-lac::ortT⁺-ghoS⁺	This study
pCA24N-ortT-B	Cm^R; lacI^q, pCA24N P_T5-lac::ortT⁺ with the BamHI site in the N-terminus linker deleted.	This study
pCP20	Ap^R; Cm^R, FLP⁺, λ cI857⁺, λ p_RRep^ts	[61]
pBS(Kan)	Km^R; cloning vector	[62]
pBS(Kan)-mqsR	Km^R; pBS(Kan) P_lac::mqsR⁺	[43]
pBS(Kan)-mqsR-mqsA	Km^R; pBS(Kan) P_lac::mqsR⁺-mqsA⁺	[43]

Cm^R and Km^R are chloramphenicol and kanamycin resistance, respectively.