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. 2015 Feb 2;7(2):380–395. doi: 10.3390/toxins7020380

Figure 2.

Figure 2

Uptake of C3 into HT22 and J774A.1 cells after inhibition of the vacuolar H(+)-ATPase on endocytosis by bafilomycin 1. HT22 cells (A) or J774A.1 cells (B) were incubated with 100 nM bafilomycin A1 for 1 h at 37 °C. Subsequently, 500 nM of C3 was applied, and the cells were further incubated at 37 °C in the presence of bafilomycin A1. In parallel, cells were incubated with C3 alone or left untreated. Cells were lysed and submitted to western blot analysis against RhoA and β-actin. The ADP-ribosylation state of Rho from cells treated with C3 in the absence or presence of bafilomycin A1 was determined by the ADP-ribosylation assay. Western blots and autoradiography from representative experiments are presented (n = 3).