Figure 3.

Uptake of C3 into HT22 and J774A.1 cells under extracellular acidic condition and in the presence of bafilomycin A1. Bafilomycin A1-pretreated HT22 cells (A) and J774A.1 cells (B) were incubated in serum-free medium with 500 nM C3 (or left untreated for control) at 4 °C. After 1 h, the medium was either adjusted to pH 5.0 or to pH 7.5, and cells were further incubated for 10 min, still in the presence of bafilomycin A1. Then, cells were incubated in neutral medium containing bafilomycin A1 at 37 °C for a further 6 h (HT22) and 4 h (J774A.1). Cells were lysed and submitted to western blot analysis probing RhoA and β-actin.