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. 2015 Feb 2;7(2):380–395. doi: 10.3390/toxins7020380

Figure 4.

Figure 4

Uptake of C3 into HT22 and J774A.1 cells after cholesterol depletion by methyl-β-cyclodextrin (MBCD). Cultivated cells ((A) = HT22 cells, (C) = J774A.1 cells) were pre-treated with methyl-beta-cyclodextrin (5 mM) for 20 min followed by incubation with C3 (500 nM) for the indicated time. Cells were lysed and submitted to western blot analysis probing RhoA and β-actin. Densitometric evaluation of RhoA is shown ((B) = HT22 cells and (D) = J774A.1 cells). Non-shifted RhoA (indicative of non-modified Rho) is quantified by densitometric evaluation and adjusted to the corresponding β-actin signal. Results represent the arithmetic means ± SD of three independent experiments. Statistical differences between C3-treated and control cells were determined using a two-sided Student’s t test (* p ≤ 0.05; ** p ≤ 0.01).