Figure 5.
Cytolytic activity of the wild type (WT) and mutants (2E3, 2EE3, 2EED3 and 2EEDE3). A total of 4 amino acids (Glutamic acid-E, Glutamic acid-E, Aspartic acid-D and Glutamic acid-E) were inserted in succession at the N-terminus. Mutants generated displayed significantly lower cytotoxic activity compared to the wild type.