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. 2015 Feb 3;7(2):407–422. doi: 10.3390/toxins7020407

Figure 5.

Figure 5

Cytolytic activity of the wild type (WT) and mutants (2E3, 2EE3, 2EED3 and 2EEDE3). A total of 4 amino acids (Glutamic acid-E, Glutamic acid-E, Aspartic acid-D and Glutamic acid-E) were inserted in succession at the N-terminus. Mutants generated displayed significantly lower cytotoxic activity compared to the wild type.