Table 3. Specific Capture of cccDNA Using Magnetic Beads Functionalized With 21-49 mer Oligonucleotides.
Probe number | Probe Length, mer | Hybridization Time, h | Concentration of Starting cccDNA, IU/mL | Detection Concentration by MCH-qPCR, IU/mLa |
---|---|---|---|---|
1 | 21 | 1 | 105 | 104 |
2 | 25 | 1 | 105 | 103 |
3 | 35 | 1 | 105 | 104 |
4 | 49 | 1 | 105 | 105 |
1 | 21 | 2 | 105 | 105 |
2 | 25 | 2 | 105 | 104 |
3 | 35 | 2 | 105 | 104 |
4 | 49 | 2 | 105 | 105 |
1 | 21 | 3 | 105 | 105 |
2 | 25 | 3 | 105 | 104 |
3 | 35 | 3 | 105 | 105 |
4 | 49 | 3 | 105 | 105 |
a Abbreviation: MCH-qPCR, magnetic capture hybridization-quantitative polymerase chain reaction.