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. 2015 Feb 22;13:71. doi: 10.1186/s12967-015-0393-4

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© Machida et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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1st screening by SEREX and Western blot analysis. Recombinant proteins were blotted onto NitroBind nitrocellulose membranes and reacted with patient plasma (arrow head). (A) Affinity-purified GST-tagged antigens were separated on 12% SDS-polyacrylamide gels stained with Coomassie staining (lane 1), or Western blotted using anti-GST antibody (lane 2) or autologous sera (lane 3) (B).