Table 6.
Shell ligand | Particle size (nm) a | Zeta potential (meV) b | Morphology c | Cargo d | Sequence e |
---|---|---|---|---|---|
Tenfibgen | 27.7 ± 4.6 | −7.4 ± 4.2 | Uniform, single capsules | siCDK11 | 5′-gagcgagcagcagcgugugdTdT-3′ |
27 kDa | |||||
Tenfibgen | 20.7 ± 5.2 | −1.7 ± 2.4 | Uniform, single capsules | siCK2 | 5′-auacaacccaaacuccacaudTdT-3′ |
27 kDa | |||||
Tenfibgen | 16.2 ± 2.4 | −7.8 ± 4.2 | Uniform, single capsules | siCON1 | 5′-uagcgacuaaacacaucaauudTdT-3′ |
27 kDa |
CDK, cyclin-dependent kinase; CK2, casein kinase 2; si, small interfering; TEM, transmission electron microscopy. aMean ± standard deviation of the average elliptical diameter determined from TEM micrographs measuring at least 20 nanocapsules. bAverage surface charge measured by dynamic light scattering from two different preparations across a 20 V potential in 1 mM KCl at 2 μg/ml. Data shown as the mean ± standard error of 15 independent measurements. cMorphology of all nanocapsules determined by visual atomic force microscopy and TEM observation as uniform, single capsules. dTBG-siCDK11, siCK2, and siCON1 encapsulation efficiencies mean of 54.0%, 86.8%, and 69.2%, respectively, observed by Burton analysis relative to unencapsulated siRNA. eSense strand sequence.