Figure 3.

Intranasal inoculation of a nonreplicating type 5 adenovirus fails to promote bacterial ascension to the chinchilla middle ear with no effect on bacterial loads detected in the nasopharynx or Eustachian tube. Chinchillas were inoculated with adenovirus strain wt300 or H5wtΔpTP (ΔpTP) 7 days prior to inoculation with Streptococcus pneumoniae. At 4 days post-bacterial-inoculation, S. pneumoniae loads were quantitated within the nasopharynx (a), Eustachian tubes (b), and middle ear bullae (c). Solid lines indicate the geometric mean bacterial CFUs for values above the dashed line, which indicates the limit of quantitation.