Figure 4.

The wild-type and E3-deleted type 5 adenoviruses promote comparable levels of Streptococcus pneumoniae ascension into the chinchilla middle ear, while the viral DNA replication-deficient virus fails to promote ascension. Middle ear samples from chinchillas euthanized at 4 (none, wt300, dl327, ΔpTP) and 7 (none, wt300, dl327) days post-bacterial-inoculation (see Table 1) were categorized as culture positive or culture negative for Streptococcus pneumoniae using the limit of quantitation as a cutoff value (2 × 10³ CFU). The number of ears (n) included in each group is indicated. The proportion of S. pneumoniae positive ears was significantly affected by the preceding virus infection (P = 0.0036, Fisher's exact test). The basis for the variation was identified by the Marascuilo post hoc test for multiple proportions and is indicated by the horizontal bars (*P < 0.05, - P < 0.075). Days 4 and 7 postinoculation results were combined for this analysis as there was no difference between middle ear incidence at these time points.