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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Dev Dyn. 2014 Nov 23;244(3):444–456. doi: 10.1002/dvdy.24221

Fig. 1. miRNAs mediate temporal gene expression during UB development.

Fig. 1

Fig. 1

In situ hybridization of Cthrc1, Hapln1, Ptprz1, and Lin28b was performed on whole-mount embryonic kidneys at E11.5 and embryonic kidney sections at E12.5–E15.5. At E11.5, all the genes are expressed in the UB epithelium of the control kidneys (a, b, c, d). At E12.5 and E13.5, except for the gene Ptprz1 (k, s), all the genes are expressed in the UB epithelium of the control kidneys (i, j, l, q, r, t). At E14.5 and E15.5, none of the genes are detected in the UB epithelium of the control kidneys (y, z, a′, b′, g′, h′, i′, j′). Expression of Cthrc1, Hapln1 and Lin28b is higher in the Dicer mutant UB epithelium at E12.5 and E13.5 (m, n, p, u, v, x) than in their control littermates, and expression in the Dicer mutant UB epithelium of Cthrc1, Hapln1, and Lin28b persists at E14.5 and E15.5 (c′, d′, f′, k′, l′, n′). Expression of Ptprz1 persists at E12.5–E15.5 (o, w, e′, m′). Arrows point to the UB epithelium. Dotted lines also delineate the UB epithelium. Scale bar = 10 μm for panels a–h; Scale bar = 5 μm for panels i–n′.