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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Dev Dyn. 2014 Nov 23;244(3):444–456. doi: 10.1002/dvdy.24221

Fig. 2. Early branching defects of Dicer UB mutant kidneys.

Fig. 2

A. The UB epithelium was visualized with GFP fluorescence from the RosamT/mG Cre reporter. (a, b) Whole-mount kidneys at E11.5 exhibit no significant difference in the first branching event between the Dicer UB mutants (b) and their control littermates (a). (c, d) Branching of the ureteric bud epithelium of the Dicer UB mutants (d) is significantly reduced compared to their control littermates at E12.5. Scale bars = 10 μm. B. UB branching morphogenesis was quantified at E12.5 by measuring the number of UB tips per kidney. The number of UB tips per kidney averaged 17.75±1.44 (n=12) for the controls and 8.95±0.50 (n=19) for the Dicer UB mutants. Statistical significance was analyzed with Student’s t-test.