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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Dev Dyn. 2014 Nov 23;244(3):444–456. doi: 10.1002/dvdy.24221

Fig. 3. Branching defect displayed by the ureteric bud epithelium of Dicer UB mutants is cell-autonomous and independent of the surrounding metanephric mesenchyme.

Fig. 3

Isolated ureteric buds from E11.5 Dicer mutants (b) and control littermates (a) showed no significant difference at the point of dissection. On culturing in Matrigel supplemented with Gdnf and retinoic acid, the mutant ureteric buds (d, f, h, j, l) displayed a significant reduction in branching compared to ureteric buds derived from kidneys of control littermates (c, e, g, i, k) as early as 24 h in the culture (d). Scale bar =10 μm.