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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Hepatol Res. 2014 Oct 7;45(7):794–803. doi: 10.1111/hepr.12411

Figure 5. Inhibition of the CXCR4/CXCL12 chemokine axis with a single AMD3100 injection in an acute CCl4 model does not prevent injury and demonstrates a trend of increased hepatic inflammation.

Figure 5

Mice received a single injection of CCl4 followed by AMD3100 12 hours later and were sacrificed between 36 and 144 hours after the CCl4 injection. Serum ALT (A) and AST (B) levels showed no significant changes between AMD3100 and control treated groups. In mice treated with AMD3100 H&E images (C) and histological scoring for necrosis (D) showed a trend of increased necrosis at 36 hours, but not at any other time points. FACS analysis of intrahepatic leukocytes similarly showed a trend of increased inflammatory cells (E), with a nearly 3-fold increase of neutrophils at 36, but not 72 hours (F). FACS analysis for hematopoietic stem cells defined as CD45+, lineage negative, Sca-1 +, c-Kit + cells (LSK) showed a significant increase in the absolute number of LSK cells at 36 hours post-CCl4 injection in mice treated with AMD3100 (G). Representative images shown, n=3-6, *P<.05.