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. 2015 Feb 25;15:93. doi: 10.1186/s12879-015-0818-y

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© Ma et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Optimized annealing temperature for sH1N1, H1N1pdm09, H3N2, H5N1 and FluB by multiplex DPO PCR. (A: Tm = 46°C; B: Tm = 50°C; C: Tm = 55°C; D: Tm = 60°C). Lanes: M: DNA Marker; 1–6: 10⁷–10² viral particles/μl. 537 bp for H3N2, 401 bp for FluB, 328 bp for H5N1, 231 bp for H1N1pdm09, and 193 bp for sH1N1.