. 2015 Feb 25;15:93. doi: 10.1186/s12879-015-0818-y

Table 1.

DPO primers used for multiplex DPO PCR

Virus	Target gene	Primer sequence (5′–3′)	Amplicon size (bp)
H1N1 pdm09	HA	F:TAGTGCTGACCAACAAAGTCTCTAIIIIIATGCAG	231
H1N1 pdm09	HA	R:GCATTTCTTTCCATTGCGAATGCIIIIITCGGTAC	231
sH1N1	HA	F:TGCGAATYACTGATTTCCAAGGAIIIIIGGTCCTA	193
sH1N1	HA	R: CTCCGGTTACRGTGTGGTGGGGIIIIIAGCTCTCT	193
H3N2	HA	F:ACGCTGTGCCTTGGGCACCATGCAIIIIIAAACGG	537
H3N2	HA	R:GTCMTTGTCCGTACCCGGGTGGTGIIIIICCCAAA	537
H5N1	HA	F:GAGAGATTGTAGTGTAGCTGGATIIIIICTCGGAA	328
H5N1	HA	R:CTTTATTGTTGGGTATGTRCTGTTIIIIITGATAAGCC	328
FluB	PB1	F:TTGGCTATGACTGAAAGAATAACCAIIIIIAGCCCA	401
FluB	PB1	R:GCATTAACAAATAGAGCAAAATCATIIIIIGATTGC	401

HA: hemagglutinin gene; PB1:polymerase basic 1 gene; F: forward primer; R: reverse primer; bp: base pair.