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. 2015 Mar 1;46:17. doi: 10.1186/s13567-015-0147-6

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© Lee et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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2DE profile of B. abortus proteins and immunoblotting with antisera from B. abortus -infected cattle. (A) 2DE profile of proteins from B. abortus detected on silver-stained 2DE gels within the pI range 4–7. Immunoblotting analyses were performed with antisera from cattle after 3 (B), 7 (C), and 10 weeks (D) of challenge with B. abortus. Three replicates of 2DE analysis were performed in the independent experiments.