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. 2015 Mar 1;46:17. doi: 10.1186/s13567-015-0147-6

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© Lee et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunoblotting profile of B. abortus proteins responded with B. abortus -infected bovine antisera excluding non-specific proteins. Immunoblotting analyses were performed with antisera from cattle after 3, 7, and 10 weeks of challenge with B. abortus. After excluding non-specific reactions, a total of 101 (A), 84 (B), and 78 (C) immunoreactive dots, as well as 55 protein spots that reacted with antisera at all 3 stages (D , E, and F), were selected and labeled. The numbers represent the serial numbers of the immunoreactive proteins in immunoblot analyses.