Table 3.
Identification of matched immunoreactive proteins of B. abortus that reacted with B. abortus -positive bovine antisera
| Spot no. | Gene name | Gene ID a | Protein identification | Protein ID a | Accession no a | Sequence length | Locus tag a | Score | M r | p I | Sequence coverage (%) | Subcellular location c | COG functional category d | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Experimental | Theorectical b | |||||||||||||
| 118 | BruAb2_0325 | 3341905 | aldehyde dehydrogenase | YP_223118.1 | Q579C7 | 500 | BruAb2_0325 | 249 | 53744 | 53435 | 5.64 | 29 | unknown | G: Carbohydrate transport and metabolism |
| 146 | BruAb2_0024 | 3341776 | branched chain amino-acid ABC transporter substrate-binding protein | YP_222837.1 | Q57A58 | 471 | BruAb2_0024 | 438 | 50740 | 44322 | 6.43 | 25 | periplasmic space | E: Amino acid transport and metabolism |
| 151 | aspC | 3339882 | aspartate aminotransferase | YP_222177.1 | Q57C18 | 400 | BruAb1_1488 | 193 | 43812 | 43554 | 5.94 | 18 | unknown | F: Nucleotide transport and metabolism |
| 161 | BruAb1_0775 | 3339474 | 3-hydroxyisobutyryl-CoA hydrolase | YP_221504.1 | Q57DZ1 | 349 | BruAb1_0775 | 258 | 38120 | 37802 | 4.84 | 27 | unknown | H: Coenzyme transport and metabolism |
| 162 | tsf | 3340671 | elongation factor Ts | YP_221867.1 | Q57CX8 | 305 | BruAb1_1167 | 367 | 32061 | 31491 | 5.03 | 35 | cytoplasm | J: Translation, ribosomal structure and biogenesis |
| 164 | mdh | 3340925 | malate dehydrogenase | YP_222574.1 | Q57AX1 | 320 | BruAb1_1903 | 76 | 33854 | 33704 | 5.24 | 7 | cytoplasm | G: Carbohydrate transport and metabolism |
| 169 | tbpA | 3340057 | thiamine transporter substrate binding subunit | YP_222421.1 | Q57BC4 | 334 | BruAb1_1742 | 310 | 36843 | 36752 | 6.06 | 19 | periplasmic space | H: Coenzyme transport and metabolism |
| 178 | BruAb1_1058 | 3341091 | cysteine synthase A | YP_221767.1 | Q57D78 | 342 | BruAb1_1058 | 417 | 34445 | 36701 | 5.94 | 30 | cytoplasm | E: Amino acid transport and metabolism |
| 187 | BruAb2_0291 | 3341871 | hypothetical protein BruAb2_0291 | YP_223086.1 | Q579F9 | 330 | BruAb2_0291 | 267 | 35457 | 35251 | 5.50 | 26 | unknown | R: General function prediction only |
| 203 | rpsB | 3340672 | 30S ribosomal protein S2 | YP_221868.1 | Q57CX7 | 256 | BruAb1_1168 | 259 | 29308 | 27999 | 5.88 | 29 | ribosome | J: Translation, ribosomal structure and biogenesis |
| 204 | ubiG | 3340925 | 3-demethylubiquinone-9 3-methyltransferase | YP_415219.1 | Q2YLN5 | 248 | BAB1_1875 | 410 | 27653 | 27486 | 5.79 | 31 | cytoplasm | E: Amino acid transport and metabolism |
| 207 | dapB | 3341712 | dihydrodipicolinate reductase | YP_223731.1 | Q576R4 | 268 | BruAb2_0991 | 536 | 28792 | 27605 | 5.92 | 45 | cytoplasm | E: Amino acid transport and metabolism |
| 218 | BruAb2_0647 | 3342272 | hypothetical protein BruAb2_0647 | YP_223419.1 | Q577X6 | 224 | BruAb2_0647 | 302 | 24961 | 24805 | 4.83 | 34 | unknown | Q: Secondary metabolites biosynthesis, transport, and catabolism |
| 227 | BruAb2_0628 | 3342294 | metal-dependent hydrolase | YP_223400.1 | Q577Z5 | 237 | BruAb2_0628 | 511 | 25223 | 25124 | 5.58 | 51 | cytoplasm | R: General function prediction only |
| 228 | msrA | 3341844 | methionine sulfoxide reductase A | YP_223747.1 | Q576P8 | 218 | BruAb2_1009 | 167 | 24230 | 24017 | 5.65 | 20 | cytoplasm | O: Posttranslational modification, protein turnover, chaperones |
| 231 | BruAb1_1470 | 3340810 | 50S ribosomal protein L25 | YP_222213.1 | Q57BY2 | 207 | BruAb1_1470 | 171 | 22369 | 22383 | 5.91 | 47 | ribosome | J: Translation, ribosomal structure and biogenesis |
| 239 | BruAb1_0588 | 3339410 | Fe-Mn superoxide dismutase | YP_221327.1 | Q57EG8 | 199 | BruAb1_0588 | 359 | 22526 | 22540 | 5.83 | 37 | unknown | P: Inorganic ion transport and metabolism |
| 240 | rocF | 3341875 | arginase | YP_223125.1 | P0A2Y1 | 306 | BruAb2_0333 | 188 | 33415 | 33182 | 5.63 | 24 | unknown | P: Inorganic ion transport and metabolism |
| 243 | gpm | 3341713 | phosphoglyceromutase | YP_223732.1 | Q576R3 | 206 | BruAb2_0992 | 446 | 22929 | 22886 | 6.16 | 43 | cytoplasm | G: Carbohydrate transport and metabolism |
| 253 | secB | 3339678 | preprotein translocase subunit SecB | YP_222709.1 | P0C125 | 163 | BruAb1_2047 | 343 | 17924 | 17878 | 4.89 | 46 | cytoplasm | O: Posttranslational modification, protein turnover, chaperones |
| 254 | ndk | 3339959 | nucleoside diphosphate kinase | YP_221449.1 | Q57E46 | 140 | BruAb1_0713 | 58 | 15269 | 15278 | 5.27 | 20 | cytoplasm | F: Nucleotide transport and metabolism |
| 257 | BruAb2_0845 | 3341366 | hypothetical protein BruAb2_0845 | YP_223598.1 | Q577E7 | 177 | BruAb2_0845 | 500 | 18506 | 18517 | 5.02 | 43 | unknown | S: Function unknown |
| 263 | ohr | 3341640 | organic hydroperoxide resistance protein | YP_223139.1 | Q579A6 | 140 | BruAb2_0347 | 8 | 14337 | 14232 | 5.63 | 13 | unknown | R: General function prediction only |
aGene ID, protein ID, accession no. and locus tag were retrieved from the NCBInr database.
bTheoretical molecular weight from the UniProtKB database entry.
cSubcellular locations were predicted using PSORTb v. 2.0.4.
dCluster of orthologous groups (COG) protein database generated by comparing microbial genomes from the NCBI COG.