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. 2015 Feb 25;15:82. doi: 10.1186/s12885-015-1065-8

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© Saito et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Changes in the phosphorylation of ERK and AKT after adding fibroblast (HEF -75hTERT) culture supernatant. In representative cell lines (TE-11, TE-14, and KYSE220), we examined the phosphorylation of ERK and AKT six-hours after the addition of supernatant. Both p-ERK and p-AKT levels are markedly up-regulated by treatment with fibroblast supernatant, though neither total AKT nor total ERK are changed. The Y axis indicates the pixel/area.