Table 1.
Islet pHi (mean ± SEM) in the presence of each secretagogue
| Nutrient | In Basal pH medium | In High pH medium |
| Glucose 16.7 mM | 7.32 ± 0.04 | 7.55 ± 0.03 |
| Leucine 20 mM | 7.09 ± 0.03 | 7.23 ± 0.04 |
| BCH 20 mM | 7.06 ± 0.03 | 7.24 ± 0.03 |
| αKIC 20 mM | 6.99 ± 0.02 | 7.18 ± 0.03 |
Islets loaded with SNARF5-AM were placed in KRBH medium of either basal pH (7.4) or high pH (8.3), containing each secretagogue. Islets were excited at 514 nm and emission fluorescence recorded at 580 and 630 nm. Average intracellular pH was calculated over a stable region of each recording, and these values from n recordings were averaged to obtain the pH values shown in the table. n = 10 for glucose; 6 for leucine and BCH; 5 for αKIC.