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. 2014 Nov 28;56(2):132–142. doi: 10.3164/jcbn.14-76

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Copyright © 2015 JCBN

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Comparison of antioxidative activities according to extract solvent. (A) Chromatography of ethanol extracts and isopropanol extracts of Artemisia. Peak of eupatilin and jaceosidin was significantly higher in isopropanol extracts compared to ethanol extracts of Artemisia. Molecular structure and chemical name was shown. (B) Higher DPPH radical reduction activity in isopropanol extracts than ethanol extracts of Artemisia. To compare the antioxidant capacity between the ethanol extracts and the isopropanol extracts of Artemisia, DPPH scavenging capability was measured. (C) Confocal imaging of DCF-DA according to extracts. The intracellular accumulation of ROS induced by LPS was measured by using DCF-DA as a probe capable of detecting peroxides. LPS caused increased intracellular accumulation of ROS in RGM1 cells, which was apparently abolished by pretreatment with isopropanol extracts. (D) The data were presented as mean ± SD. for three different experiments performed in triplicate.