Fig. 2.

Comparison of HO-1 expression according to extraction solvent. Isopropanol extracts induces HO-1 protein (A) and mRNA expression (B). HO-1 protein and mRNA expression were increased following Artemisia extracts treatment in a dose-dependent manner irrespective of extraction solvent. However, 30 µg/ml isopropanol extract showed significantly increased expression of HO-1. HO-1 protein (A) and mRNA (B) expression were analyzed by Western blotting and RT-PCR, respectively. (C) Cell viability. Artemisia extracts were tested for their cytotoxic activity using the MTT colorimetric assay. The data were presented as mean ± SD. for three different experiments performed in triplicate.