Fig. 1.

Effects of NADPH oxidase inhibitors on reporter gene activity in DLD-1/COX-2-B2-βGal-BSD cells. DLD-1/COX-2-B2-βGal-BSD cells were seeded in 96-well multi-well plates at a density of 2.0 × 10⁴ cell/well and cultured in medium containing the indicated NADPH oxidase inhibitors, apocynin (A), benzaldehyde (B) and vanillin (C), at concentrations up to 800 µM for 48 h. After 48 h, the COX-2 transcriptional activity was evaluated by β-galactosidase activity and was normalized for viable cell numbers assessed by MTT assay. The columns indicate the values of the mean percentages of triplicate wells of transcriptional activity of DLD-1/COX-2-B2-βGal-BSD cells. The data are representative of more than three independent experiments. Data are mean ± SD (n = 3). *p<0.01 vs 0 µM.