Fig. 2.
Expression levels of components of NADPH oxidase in human colon cancer cells with or without sesamol treatment. DLD-1/COX-2-B2-βGal-BSD cells were seeded in 24-well multi-well plates at a density of 2.0 × 105 cell/well and cultured in medium containing 50 and 100 µM sesamol for 48 h. After 48 h treatment, quantitative real-time PCR analysis was performed to determine NOX1 (A), NOX2 (B), p22phox(C) mRNA levels. Data are normalized with GAPDH. The data are representative of more than three independent experiments. Data are mean ± SD, n = 3. *p<0.01, **p<0.001 vs 0 µM. After indicated dose and time treatment of sesamol, Western blot analysis was performed to determine NOX1 (D) protein levels. β-actin was proved as a loading control. Densities of the bands were calculated and are shown under the band as % of control. NOX; nicotinamide adenine dinucleotide phosphate oxidase.