Immunohistochemical detection of A2AR in synovial membrane. Immunohistochemical analysis of synovial tissue from CIA-control mice revealed positive staining for A2AR in contrast to Sham-control mice (b, a). The staining for A2AR in CIA-EA mice was weaker than in CIA-control mice, but the difference was not significant (c, b). However, the staining for A2AR was significantly increased in CIA-EA-SCH58261 mice (d, c) and in CIA-SCH58261 mice compared to the CIA-control mice (e, b). Values (IOD in %) are means ± SEM of 10 animals for each group. *
P < 0.05 versus Sham-control. #
P < 0.05 versus CIA-EA (f). Magnification ×400 (a, b, c, d, and e).