Figure 4.

Immune responses against the 10-vPMO cocktail after the long-term systemic treatment.(a) Immunostaining against CD3 antigen (red) of a pan T cell marker in diaphragm (DIA), biceps femoris (BF), quadriceps (QUA) and heart (HRT) muscles from wild-type at 6-month-old, nontreated and treated mdx52 mice 2 weeks after the final i.v. injection of the vPMO cocktail. Nuclei (blue) were counterstained with DAPI. Scale bar, 100 μm. (b) Counting CD3-positive cells on the muscle sections. The number of sporadic CD3-positive cells was counted on 10 section areas at random through a 20× objective lens. Data (n = 4 in nontreated and n = 6 in treated group) are presented as mean ± SD.