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. 2015 Feb 17;4(2):e227. doi: 10.1038/mtna.2015.3

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Protection of sh1005-modified splenocytes. Comparison of p24 reactivation in sh1005 (EGFP+) and no shRNA (mCherry+) population ex vivo. Splenocytes were isolated from R5-tropic HIV-1_NFNSX–challenged hu BLT mice (n = 7) or X4-tropic HIV-1_NL4-3–challenged hu BLT mice (n = 7). Splenocytes were isolated, stimulated with PHA/IL-2 for 5 days to reactivate HIV-1 and stained with anti-HIV-1 p24 gag monoclonal antibodies. (a) Representative p24 intracellular staining data in CD45+CD3+CD8- cell population were shown. (b) Results of p24 reactivation in EGFP+ population (solid circle) and in mCherry+ population (open circle) from all analyzed mice are shown. The percentages of p24 expression within EGFP+ or mCherry+ populations were normalized based on % each marker (EGFP or mCherry) expression for the comparison. *P ≤ 0.05. n.s., not significant.