Figure 2. HIV-1 Nef and Vpu reciprocally regulate NRON expression.

(A) Relative NRON expression levels at different times after infection of Jurkat cells with 1 moi of HIV-1 NL4-3 and HIV-1 NL4-3ΔNef viruses. The NRON levels were estimated by quantitative RT-PCR, were normalized to Actin and are expressed relative to levels found in mock-infected Jurkat cells. The cropped western blots show Nef and Vpu expression at different times after infection with HIV-1 NL4-3, with Actin as a loading control; the full images for these are shown in Fig. S7B. (B) Relative NRON expression levels at different times after infection of U937 cells with 1 moi of HIV-1 NL4-3 and HIV-1 NL4-3ΔNef viruses. The NRON levels were estimated by quantitative RT-PCR, were normalized to actin and are expressed relative to levels found in mock-infected U937 cells. (C) NRON expression levels in U937 cells stably expressing the Nef-EYFP fusion protein compared to cells stably expressing EYFP, at 48 hr after plating. (D) NRON expression levels in U937 stably expressing the Vpu-GFP fusion protein compared to cells stably expressing GFP, at 48 hr after plating. Results from three separate experiments are shown as mean values ± SD; * p<0.05.