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. 2015 Mar 2;5:8670. doi: 10.1038/srep08670

Figure 3. The sigma-1 receptor is expressed in HSPCs and mediates cocaine's hematosuppressive effects.

Figure 3

(A) RT-PCR analysis of sigma-1 receptor in FL derived HSPCs from four separate donors. S1R expression is graphed relative to GAPDH expression and the average of three replicate reactions is plotted. (B) Methylcellulose colony forming assays of FL derived HSPCs that were either untreated, cocaine treated, S1R block treated, and cocaine + block treated. Each mark represents the score on a single plate, and the bar marks the mean. (C) Representative images of hematopoietic colonies that developed from S1R-block treated (top) or untreated (bottom) HSPC. (D) Phenotypic analysis of colonies that developed from HSPCs that were either untreated, cocaine treated, S1R block treated, and cocaine + block treated. Red boxes/E erythroid colonies, blue boxes/G granulocyte colonies, black boxes/M macrophage colonies, green boxes/GM mixed granolocyte/macrophage colonies.