. 2015 Mar 2;5:8663. doi: 10.1038/srep08663

Table 1. Total sonication energy input versus cell suspension volume for maximal CFPS productivity.

Strain	Cell suspension volume (μL)	Sonication energy (J)
Strain	Cell suspension volume (μL)	Predicted energy input for >95% productivity	Predicted energy input for 100% productivity	Observed energy input for 100% productivity	Difference (%)^a
BL21 Star™ (DE3)	100	24 ± 11	37	24	54.2
	300	143 ± 39	150	140	7.1
	500	299 ± 71	263	309	15.0
	750	426 ± 72	404	398	1.5
	1000	639 ± 206	545	532	2.4
	1500	798 ± 105	826	822	0.5
K12 MG1655 C495	100	23 ± 6	−10	26	138.0
	300	146 ± 32	145	138	5.0
	500	290 ± 38	300	285	5.1
	700	417 ± 143	454	414	9.7
	1000	613 ± 151	686	705	3.0
	1500	940 ± 230	1073	1080	3.0

(a)Energy differences between predicted and observed 100% productivity.