Figure 1. Memory formation and maintenance in 10 dpf zebrafish larvae, measured by visual lateralization in response to a novel object.
Results are presented as mean values over 2 min intervals T0, 0–2 min; T1, 2–4 min; T2 4–6 min; T3, 6–8 min giving a total of 8 minutes per session. LES or RES preferences are marked by significance above the bars in the graphs. (a) RES use when presented with a novel object during the familiarization phase. (b) RES use when novel objects are reintroduced 1 hour after familiarization. Significant RES preference was detected at T3 (t = 6.78, p < 0.001). (c) Mean RES use when viewing a novel object when reintroduced 2 hours post familiarization. Significant RES preferences are observed at T0 (t = 3.09, p = 0.003) and T2 (t = 2.46, p = 0.018). (d) RES use when novel objects are reintroduced 3 hours after initial familiarization. A significant LES preference is detected at T0 (t = −2.52, p = 0.016) and RES preferences at T2 (t = 3.32, p = 0.002) and T3 (t = 4.46, p < 0.001). Significance *p < 0.05, **p < 0.001, ***p < 0.0001, computed using RMANOVA and Turkey's post-hoc test or a single sample t-test, tested against 50%. n = 48. Error bars indicate standard error of mean (SEM).