TABLE 1.
TaqMan qPCR oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ to 3′) | Target | Size (bp) | Reference |
|---|---|---|---|---|
| AB1242 | AAAACAGGCTTACCGCTCCAA | opag2 | 151 | 32 |
| AB1243 | GGCGTGTAGCTAGGCTCAAAGT | |||
| AB1250a | CTCTCCTCTGCTCAGGGCTCTGCG | |||
| AB1345 | GGTGACCGTAAGGCTTGATG | aadA | 279 | This study |
| AB1346 | ACCAAGGCAACGCTATGTTC | |||
| AB1347b | ACCATTGTTGTGCACGACGACA | |||
| nMSP5F | AAGTTGTAAGTGAGGGCATAGCCTCC | msp5 | 188 | 25 |
| nMSP5R | AACTTATCGGCATGGTCGCCTAGT | |||
| RFPF | TACGGTGACGCAGGATTCATCACTG | mcherry | 227 | This study |
| RFPR | ATGTCGTCTTGACCTCGGCATCAT |
a
Oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5′ end and tetramethylrhodamine (TAMRA) at the 3′ end.
b
Oligonucleotide labeled with tetrachlorofluorescein (TET) at the 3′ end and black hole quencher 1 (BHQI) at the 3′ end.