Table 5.

Effect of combining the mutation in exonuclease domains of DNA polymerases δ and ε genes with active site mutation in DNA polymerases α and δ genes on mutation rates in mismatch repair proficient haploid derivatives of 8C-YUNI101

Relevant mutation	Mutation rates
	Forward Canr mutation		Ura+ reversion (base substitutions)		His+ reversion (+ 1 frameshifts)
	Rate (× 10-7)	Fold increase	Rate (× 10-8)	Fold increase	Rate (× 10-8)	Fold increase
no	4.4	1	1.3	1	1.9	1
	2.9–5.8		1.2–1.7		1.4–3.6
pol1-Y869A	12	2.7	1.1	0.8	6.4	3.3
	6.2–28		0.8–2.2		4.1–16
pol2-4	17	3.8	5.1	3.9	51	27
	12–24		3.7–5.5		45–71
pol2-4 pol1-Y869A	46	10	8.8	6.7	120	63
	41–58		4.6–11		77–160
pol3-01	190	43	19	15	110	59
	136–260		6–35		80–160
pol3-01 pol1-Y869A	220	50	34	26	114	60
	140–240		27–48		87–210
pol3-Y708A	28	6.3	6.1	4.7	6.0	3.1
	14–32		5.0–7.7		4.8–11
pol2-4 pol3-Y708A	92	21	15	12	73	38
	73–120		14–18		61–104