FIG 3.

Flow cytometric (A) and whole-cell ELISA (B) analyses of Lactobacillus sp. cells after incubation with DARPin I_07-cA fusion-containing growth medium and detection with FITC-conjugated human IgG (gray bars). MFI and A₄₅₀ values were normalized to those for the control samples (white bars). (C) Quantification of LTA on the surfaces of Lactobacillus spp. by whole-cell ELISA using an LTA-specific monoclonal antibody. ACID, Lb. acidophilus ATCC 4356; BULG, Lb. delbrueckii subsp. bulgaricus ATCC 1184; CASE, Lb. casei ATCC 393; GASA, Lb. gasseri ATCC 33323; GASK, Lb. gasseri K7; PARA, Lb. paracasei ATCC 25302; PLAN, Lb. plantarum ATCC 8014; REUT, Lb. reuteri ATCC 55730; RHAM, Lb. rhamnosus ATCC 53103; SALI, Lb. salivarius ATCC 11741. *, significant increase in IgG binding (t test; P < 0.05) in comparison to control.