FIG 2.
Response of NO2− production by N. viennensis and N. gargensis when exposed to C2 to C10 1-alkynes. Cultures were harvested and aliquots of cell suspensions were added to treatments containing their respective media with 10 μM Caq of C2 to C10 alkyne and 1 mM NH4+. N. viennensis and N. gargensis were incubated at 42 and 46°C, respectively, and NO2− accumulation was measured. Experiments were repeated at least three times with similar results. Bars represent the average activity in three replicate treatments compared to the no-alkyne control. Error bars represent the standard deviations of the average.