TABLE 2.
Primers and probes sequences used in this study for mRNA measurementsa
| Target mRNA | Primer or probe | Sequence (5′–3′) |
|---|---|---|
| TBP | FW | TTGTACCGCAGCTTCAAAATATTGT |
| RV | GCAGCAAATCGCTTGGGAT | |
| TP | FAM-TTGACCTAAAGACCATTGCACTTCGTGCA-TAMRA | |
| B2M | FW | CCACTGAGACTGATACATACGCCT |
| RV | GATCACATGTCTCGATCCCAGTAG | |
| TP | FAM-TAAGCATGCCAGTATGGCCGAGCC-TAMRA | |
| SDHA | FW | ACATCAGAACTACGCCTAAACATGC |
| RV | AAACCATTCCCCTGTCGAATG | |
| TP | FAM-AACCATGCTGCAGTGTTCCGTGTGG-TAMRA | |
| RPL13A | FW | GCGCCTCAAGGTGTTGGAT |
| RV | CCCAGGTAAGCAAACTTTCTGG | |
| TP | FAM-TGGTCCCTGCTGCTCTCAAGGTTGTT-TAMRA | |
| HPRT | FW | TTATCAGACTGAAGAGCTACTGTAATGATC |
| RV | TTACCAGTGTCAATTATATCTTCAACAATC | |
| TP | FAM-TGAGAGATCATCTCCACCAATAACTTTTATGTCCC-TAMRA | |
| PPIA | FW | GCCGCGTCTCCTTCGAG |
| RV | GTAAAGTCACCACCCTGGCAC | |
| TP | FAM-TTGCAGACAAAGTTCCAAAGACAGCAGAAA-TAMRA | |
| β-Actin | FW | AGAGGGAAATCGTGCGTGAC |
| RV | CAATAGTGATGACCTGGCCGT | |
| TP | FAM-CACTGCCGCATCCTCTTCCTCCC-TAMRA | |
| GAPDH | FW | TCACCACCATGGAGAAGGC |
| RV | GCTAAGCAGTTGGTGGTGCA | |
| TP | FAM-ATGCCCCCATGTTTGTGATGGGTGT-TAMRA | |
| IL-6 | FW | GAGGATACCACTCCCAACAGACC |
| RV | AAGTGCATCATCGTTGTTCATACA | |
| TP | FAM-CAGAATTGCCATTGCACAACTCTTTTCTCA-TAMRA | |
| IL-10 | FW | GGTTGCCAAGCCTTATCGGA |
| RV | ACCTGCTCCACTGCCTTGCT | |
| TP | FAM-TGAGGCGCTGTCATCGATTTCTCCC-TAMRA |
a
TBP, TATAA box binding protein; B2M, β2-microglobulin; SDHA, succinate dehydrogenase complex subunit A; RPL13A, ribosomal protein L13a; HPRT, hypoxanthine phosphoribosyltransferase; PPIA, peptidyl-prolyl cis-trans-isomerase A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-6, interleukin-6; IL-10, interleukin-10; FW, forward primer; RV, reverse primer; TP, hydrolysis probe; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.