TABLE 1.

Primers used to generate derivatives of L. reuteri TMW1.656 by double-crossover mutagenesis

Primer name	Primer sequence (5′–3′)
N1	AAGGATCCGGTTGTTGGAAGAGGATTTGAA
N2	TATAGAGTCGACTTAACTAGTAAGATTCATTGATAACATCCTT
N3	ATAACAGTCGACAAGGAGCATTAATTTCCATGAAA
N4	AACTGCAGTGTTCAGGAATGATTTTTGAGG
P1	GCAGGATCCCGACTTGGAGGAATATGGAA
P2	AACGGCGTCGACGTTAGACATTATCCCAATATCAGTCATTAT
P3	GCATTAGTCGACTTTATCTAAAGGAAGTTATAGTTATGACTGAAAA
P4	TGCTGCAGCACGTGCAATATTTCCACCA
T1	TTAGGATCCGGAAGAATGGCAGATTCCAA
T2	ATTCGAGTCGACTTATCCTTTCATCGGTACCTTTAATC
T3	ACGGATGTCGACTCTAAATCGTAATATTTATGCGTAATTACA
T4	GCCTGCAGGGGTTCAAATCCCCTTGTCT
R1	ATTAAGCTTGGTCCAATAATTGCAAAACCA
R2	ATTGGATCCTAACATTCTATTGGTCATTTATTGATTCTC
R3	GCAGGATCCAATGACTGATATTGGGATAATGTCTTA
R4	ACTTGAGTCGACATTGCTGTTGACCCAGAACC
A1	TGAGGATCCTGGACCATTTTCTGCAGTTG
A2	TTCAATGTCGACCTAATTCATTGGCTCATCAAG
A3	TCCGCTGTCGACGTAATTTAGACTAATAACGGGCTTGATG
A4	TATAAGCTTCTTTGCGCATAAGGATGACA