TABLE 4.
Intergenic mutations identified in the 2,000th generation acid-adapted clonesa
| Proximal gene(s) | Mutation (positions, nucleotide change) | Identification of a mutation in clone: |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| H9-1 | H9-2 | B11-1 | B11-2 | F11-1 | F11-2 | F9-2 | F9-3 | ||
| matP →/← ompA | Intergenic (+40/+36, C to A) | X | |||||||
| racR ←/→ ydaS | Intergenic (−61/−63, C to A) | X | |||||||
| Y75_p4289 ←/→ eaeH | Intergenic (−545/−1035, C to A) | X | |||||||
| proL → | Noncoding (57/77 nt, C to A) | X | X | ||||||
| nuoA ←/← lrhA | Intergenic (−87/+544, C to A) | X | X | ||||||
| yfjL ←/← yfjM | Intergenic (−258/+102) +8 bp (GCACTATG) | X | |||||||
| rhaA →/→ rhaD | Intergenic (+308/−143, C to A) | X | X | ||||||
| rrsC ← | Noncoding (1417/1542 nt, +A) | X | X | ||||||
| pabA →/→ argD | Intergenic (+34/−52, A to G) | X | X | ||||||
| yjbI →/→ ubiC | Intergenic (+84/−139, G to T) | X | X | ||||||
| dcuA ←/← aspA | Intergenic (−202/+104, G to T) | X | X | ||||||
| acs ←/→ nrfA | Intergenic (−228/−165, G to A) | X | X | ||||||
a
The mutations listed indicate intergenic differences between the adapted clones and the ancestral wild type. A positive distance from a proximal gene represents the downstream location of the mutation relative to the gene's translation stop site, whereas a negative distance represents the mutation's upstream location relative to the gene's translation start site. nt, nucleotide position number.