FIG 4.

Effects of amino acids on SCH814 growth in vivo and in vitro. (A) Selected amino acids at 4 mM (final concentration) were individually added to M9 medium with glucose. After 24 h of incubation at 30°C, the cells were subjected to optical density determinations at 600 nm. (B) A. stephensi mosquitoes were fed 10% sucrose supplemented with SCH814 for 24 h (NanoLuc reporter strain). After the adult mosquitoes emerged, they were fed 10% sucrose with 10 mM arginine or 10% sucrose without arginine. After 24 h and 72 h, 30 mosquitoes were randomly sampled from sucrose without arginine treatment at each time point. Under the same conditions, 28 and 30 mosquitoes were sampled from sucrose with 10 mM arginine treatment, respectively. Mosquitoes were homogenized and subjected to NanoLuc assays. Significant differences between arginine addition and no-arginine addition samples were determined by using PROC GLM; significantly different cell densities are denoted by an asterisk (P < 0.05).