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. 2015 Feb 27;81(6):2215–2225. doi: 10.1128/AEM.03110-14

FIG 2.

FIG 2

(A) Methanol consumption in CGXII defined medium supplemented with 55 mM glucose and 120 mM methanol. (B) Growth in CGXII defined medium supplemented with 55 mM glucose. (C) Growth in CGXII defined medium supplemented with 55 mM glucose and 120 mM methanol of recombinant C. glutamicum strains. Induction of gene expression was achieved with 1.5 mM IPTG in the case of Ptac-controlled gene expression. Three independent shake-flask experiments, always accompanied by a methanol evaporation control, were performed for the determination of the methanol concentration (◇). Monitoring of growth was performed in 48-well FlowerPlates by using a BioLectorBasic apparatus (n = 3). Strains: C. glutamicum Bm(mdh-act) Bs(hps-phi) (■), C. glutamicum Ptuf-Bm(mdh-act) Ptuf-Bs(hps-phi) (●), C. glutamicum Ptuf-Bm(mdh-act) pEKEx2 (▲), C. glutamicum pVWEx2 pEKEx2 (+ IPTG) (□), and C. glutamicum pVWEx2 pEKEx2 (- IPTG) (○).