TABLE 3.
Cell dry weight measures for C. glutamicum strainsa
| Strain | CDW (mg/ml of culture broth) |
|---|---|
| Recombinant C. glutamicum strains | |
| Bm(mdh-act) Bs(hps-phi) | 6.72 ± 0.05 |
| Ptuf-Bm(mdh-act) Ptuf-Bs(hps-phi) | 8.37 ± 0.03 |
| Control (pVWEx2 pEKEx2) | 6.49 ± 0.13 |
| Recombinant C. glutamicum Δald ΔadhE strains | |
| Ptuf-Bm(mdh-act) Ptuf-Bs(hps-phi) | 6.75 ± 0.01 |
| Ptuf-Bm(mdh3-act) Ptuf-Bs(hps-phi) | 7.14 ± 0.13 |
| Ptuf-Bm(mdh-act) pEKEx2 | 4.87 ± 0.25 |
| Control (pVWEx2 pEKEx2) | 6.35 ± 0.10 |
a
Cells were grown in CGXII defined medium containing glucose (55 mM) and methanol (120 mM). The CDW was determined 32 h after inoculation (the second growth phase). Genes under the control of Ptac were induced with 1.5 mM IPTG at an OD600 of 1.0. Means and standard deviations were calculated from triplicates.