Fig. 2. Talin changes the topology of the β3 TMD. A peptide containing the β3 TMD and cytoplasmic domain was labeled with environment sensitive bimanes at the outer edge of the TMD (N terminal labeling, Leu⁶⁹⁴) or at the TMD cytosol interface (C-terminal labeling, Ile⁷²¹). The peptides were individually embedded in phospholipid nanodiscs and increasing concentrations of talin head domain (THD) were added and bimanes emission spectra were recorded. The increased fluorescence indicates that both sides of the β3 TMD were in a less polar environment suggesting that THD increased the tilting of the β3 TMD. Adapted from reference (41).